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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which leads to chromosome instability, and therefore may well add to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants were reported but comprehensive nucleotide sequence info isn't yet obtainable.
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Blocking peptides are peptides that bind especially on the concentrate on antibody and block antibody binding. These peptide normally contains the epitope acknowledged through the antibody.
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This guide outlines further more attainable optimization of cellular and immunoassay parameters to be certain the best possible final results are...
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This guide outlines further feasible optimization of cellular and immunoassay parameters to ensure the very best final results are...
Antibodies sure to the blocking peptide no longer bind into the epitope within the goal protein. By comparing the staining with the blocked antibody compared to the antibody alone, you can see which staining is precise.
Focus on Facts Cyclin E belongs into the remarkably conserved cyclin website family members, whose customers are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show unique expression and degradation designs which add to your temporal coordination of each mitotic occasion. Cyclin E varieties a posh with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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